Resumo

HERNANDEZ, Erick et al. Prevalence of β-lactamase CTX-M-15 in phylogenetic groups of uropathogenic Escherichia coli isolated from patients in the community of Merida, Venezuela. Invest. clín [online]. 2014, vol.55, n.1, pp.32-43. ISSN 0535-5133.

In this study we determined the prevalence of extended-spectrum b-lactamases (ESBLs) in phylogenetic groups of uropathogenic E. coli (UPEC) isolated from patients in the community. Twenty one UPEC strains with reduced susceptibility to broad-spectrum cephalosporins were collected between January 2009 and July 2010, from patients with urinary tract infection who attended the Public Health Laboratory in Mérida, Venezuela. Genotypic characterization determined that all UPEC strains harbored bla_BLEEs genes: 76.2% of the strains showed the presence of a single ESBL-producer gene, represented by bla_CTX-M-15, whereas 23.8% of UPEC showed various combinations of bla genes (bla_CTX-M-15 + bla_TEM-1, bla_CTX-M-15 + bla_SHV and bla_SHV + bla_TEM-1). In this study, 61.9% of the isolates were placed in phylogroup A and the remaining strains were assigned to group B2 (38.1%). There was no evidence of spread of a particular UPEC clone; only seven strains belonged to a clonal group with an index of similarity greater than 85%. To our knowledge, this is the first description of bla_{CTX-M-15 in UPEC from patients with community-acquired urinary tract infections, which shows that Venezuela is also part of the so-called CTX-M-15 pandemic. The findings in this study, as well as its clinical and epidemiological implications, lead to the need for monitoring and controlling the spread of CTX-M-15 producing UPECs, not only regionally, but also nationwide.}

Palavras-chave : Escherichia coli; uropathogen; beta-lactamases; CTX-M-15; Venezuela.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )