Abstract

TERAN-ANGEL, Guillermo et al. Design of an in-house diagnostic test for IgG antibodies against hepatitis C virus detection: Incorporation of envelope protein E2 and non-structural protein 2 ( NS2). Invest. clín [online]. 2017, vol.58, n.2, pp.154-167. ISSN 0535-5133.

During hepatitis C (HCV) infection specific antibodies appear several weeks after exposure, including viral envelope (E2) and non-structural protein 2 ( NS2). In this work we designed an in-house ELISA assay, that incorporate beside NS3, NS5a, NS5b and core, the HCVE2 and NS2 proteins in substitution of NS4, in order to evaluate its diagnostic utility as compared to a fourth generation commercial kit. The in-house test demonstrated a specificity and sensitivity similar to those obtained with the commercial kit, with a kappa index equal to 0.969, when it was compared with the 4th generation commercial kit (BioelisaBiokit ©), suggesting that our test could be used for the diagnosis of specific IgG antibodies detection against hepatitis C and to become a more economic national alternative.

Keywords : hepatitis C; HCV; E2; NS2; ELISA; serological diagnosis.

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