Abstract

CASTRO BARRIOS, Eilyn Carolina et al. Polymorphism -308 of TNFα gene and its local expression in preinvasive lesions and cervical cancer in Zulia state, Venezuela. Invest. clín [online]. 2020, vol.61, n.4, pp.335-348.  Epub Feb 22, 2021. ISSN 0535-5133.  https://doi.org/10.22209/ic.v61n4a04.

Cervical cancer (CC) is a public health problem worldwide, and it represents the first cause of mortality in Venezuelan women between 25 to 44 years of age. The tumor necrosis factor alpha (TNFα) is a proinflammatory cytokine. Its expression is at the transcriptional level and some polymorphisms have been associated with the progression of some types of cancer, among these, the CC. The objective of the present study was to characterize the -308 polymorphism of the TNFα gene and its local expression in preinvasive lesions and CC. Peripheral blood DNA samples and cervical biopsies from 8 healthy women and 33 with cervical lesions were analyzed, DNA was genotyped by PCR-RFLP using restriction enzymes (NcoI), and immunohistochemical labeling was used for its expression in tissues. The -308 polymorphism of the TNF-α gene was found in Hardy-Weimberg equilibrium for both, individuals and lesions, as well as for healthy individuals. It was found that the most frequent genotype was AA (60.6%), while the genotype A/G accounted for 39.4% of the total patients with lesion and no G/G genotype was detected. TNF expression is by cellular cells in heterogeneous tissues, statistically significant differences were observed between the inflammatory group and CIN II (p <0.045). The results reveal an association between the A allele of the -308 polymorphism of the TNFα gene and the development of preinvasive lesions, with an OR = 1.538, with a confidence interval of 95% ranging from 0.32-7.26 with a p = 0.58.

Keywords : cervical cancer; TNFα; -308 polymorphism of TNFα gene; cervical intraepithelial neoplasia.

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