Resumen

ZENG, Xin et al. Induced differentiation of adipose-derived stem cells enhance secretion of neurotrophic factors. Invest. clín [online]. 2023, vol.64, n.3, pp.267-280.  Epub 10-Sep-2023. ISSN 0535-5133.  https://doi.org/10.54817/ic.v64n3a01.

Adipose-derived stem cells (ADSCs) could be ideal seed cells for repairing nerve injury as they have the potential for multidirectional differentiation. However, it is still unclear whether the undifferentiated or the differentiated ADSCs have priorities in promoting axonal regeneration and myelin formation. In this study, the primary ADSCs from rats were cultured and differentiated. The morphology, differentiation potential, and secretion of neu rotrophic factors of ADSCs were compared before and after induction. Undifferentiated ADSCs (uADSCs) were aggregated into bundles containing reticular, star, and polygonal structures. They contained a large number of lipid droplets and were positive for Oil red O staining. After differentiation, differentiation ADSCs (dADSCs) become long and spindle-shaped with decreasing protrusions around the cells, spiraling growth, and were negative for Oil red O staining. When comparing the groups the flow cytometer analysis showed: similar CD29 and CD45 surface markers in both groups; and CD44 and CD90 markers were very low in the undifferentiated groups. The levels of neurotrophin 3 (NT-3) and neuregulin 1 (NRG-1), and their receptors tropomyosin receptor kinase C (TrkC) and receptor protein-tyrosine kinase erbB-4 (ErbB-4) in dADSCs were higher than those in uADSCs. While the expressions of myelin protein zero (P0), myelin-associated glycoprotein (MAG), and purine receptor P2X7 (P2X7) were not significantly different before and after differentiation. It may be speculated that the dADSCs have enhanced abilities in nerve repairment which is associated with increased expression of neurotrophic factors.

Palabras clave : differentiated ADSCs; Schwann cells; neurotrophic factors; P2X7; nerve damage.

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