Resumo

DIAZ, M et al. Sensibilidad a Citostáticos de Células de Tumores Cerebrales Humanos en Cultivo. AVFT [online]. 2002, vol.21, n.2, pp.177-182. ISSN 0798-0264.

Malignant glioma cell cultures have been done for studying the biology of the tumors, the efficiency of antineoplasic drugs, and for determining the differential sensitivity to cytostatics. Fourteen patients with clinical and radiological diagnostics of malignant brain tumor were selected according to specific criteria, including the evaluation by Karnofsky scale, which determines the functional independence of the patient. After surgical resection of tumors, histopathological study was done and a sample was taken for monolayer cultures. Confluence was reached 15 to 45 days after plating and the cultures were exposed to (1,3-bis(2-chloroethyl)-1-nitrosourea, cisplatin or vincristine for one hour. Integrity of cell membranes was evaluated by the exclusion of Tripan blue. There were statistical significant differences between drugs and between the tumors, including those with the same histophatological diagnostic, indicating a differential resistance of the tumoral cells to the cytostatics used. The results of this study support the viability for further evaluations in order to suggest specific antineoplasic treatments probably in combination with radiotherapy for patients having malignant brain tumors.

Palavras-chave : Brain tumors; Sensitivity to cytostatics; Tumor cultures.