Abstract

MEDINA MARTEL, Matilde; FAZZINO, Fili  and  LIMA, Lucimey. Efecto de la restricción física sobre el papel de los receptores 5-HT1A en la proliferación linfocitaria. AVFT [online]. 2012, vol.31, n.4, pp.88-93. ISSN 0798-0264.

Stress affects the immune system, however, little is known about the effects on specific modifications of lymphocytes serotonin receptors. The effects of restraint stress on the role of 5-HT_1A receptors in lymphocyte proliferation were evaluated in male adult rats. They were placed in Plexiglass boxes, during 5 daily hours for 1, 3 or 5 consecutive days. Next day a blood sample was obtained by cardiac punction. Lymphocytes were isolated by density gradient and adhesion to plastic. They were cultured with agonist 8-hydroxy-di-n-propyl-aminotetralin (8-OH-DPAT) or antagonist N-(2-(4-(2-methoxyphenyl)-1-piperazinyl) ethyl)-N-(2-pyridiyl) cyclohexanecarboxamide (WAY-100635) of 5-HT_1A receptors and the mitogen concanavalin A. Proliferation was measured by tetrazolium salts. 8-OH-DPAT did not modify cell proliferation; WAY-100635 diminished it. Restraint stress increased the susceptibility to effect of WAY-100635. These results suggest changes in the expression or functional modulation of 5-HT_1A receptors in lymphocytes by stress, similar to previous reports on serotonergic system in rat brain.

Keywords : stress; 5-HT_1A receptors; lymphocytes.