Abstract

BRUGES, GA et al. Clonamiento Molecular de las Asas Intracelulares de los receptores Muscarínicos M2 y M3 implicadas en la interacción con Proteínas G en el Músculo Liso Traqueal de Bovino. RFM [online]. 2002, vol.25, n.1, pp.48-50. ISSN 0798-0469.

Two muscarinic receptors subtypes (mAChRs), m2 and m3, have been reported to regulate in opposite ways the activity of a membrane-bound guanylyl cyclase (GCm) via G-proteins in bovine tracheal smooth muscle (BTSM). The present study was undertaken to identify the primary sequences from intracellular loops of mAChRs subtypes from BTSM involved in the interaction with these G-proteins. cDNA from BTSM was used to amplify via RT-PCR, by using specific primers, the intracellular regions of m2 and m3 involved in the interaction with G-proteins. Amplification products for m3 and m2 loops were readily identified in agarose gel electrophoresis. Sequences for these loops were almost identical (99%) those found in bovine brain m2 and m3 mAChRs. This is the first molecular cloning of m2 and m3 mAChRS for BTSM. These results suggest the existence of similar mAChRs/G-proteins interactions in bovine airways.

Keywords : Muscarinic receptors; Smooth muscle; Guanylyl cyclase.