Abstract

DE MARCUCCI, O; ESCALONA, I  and  ESPINOZA, J. Inmunotitulación del Complejo Piruvato Deshidrogenasa en Hígado de rata durante un régimen de Inducción-Represión. RFM [online]. 2002, vol.25, n.1, pp.63-66. ISSN 0798-0469.

The effects of nutrients on the amount of pyruvate dehydrogenase complex (PDC) present in hepatic mitochondria were investigated in Sprague-Dawley rats fed on a high-sucrose diet. After 21 days of feeding on a high-sucrose fat-free diet (SLG), the animals were separated in two groups: one continued on the SLG diet (induction) and the other received the same diet supplemented with 10% fish oil (repression). After 3 weeks, mitochondrial PDC activity was titrated against anti-rat PDC Ig G to establish the amount of protein required to neutralize 200 mg of IgG (PE, equivalence point). The SLG diet produced an increase in the total activity of the complex and a decrease in PE as compared with control animals. Fish oil feeding produced the opposite results. Results indicate that immunetitration is a sensitive method to detect changes in the expression levels of PDC due to dietary manipulation.

Keywords : Pyruvate dehydrogenase complex; Immunetitration; Sucrose feeding.