Resumen

SANCHEZ DE V, S et al. Efecto de Péptidos Natriuréticos sobre la actividad de la Guanililciclasa asociada a las Membranas Plasmáticas de Vías Aéreas de Bovino. RFM [online]. 2002, vol.25, n.1, pp.83-86. ISSN 0798-0469.

In the plasma membranes obtained by fractionation of smooth muscle bovine airways, a guanylyl cyclase activity has been described which is subjected to regulation by muscarinic agonists and inhibited by NaCl. This latter effect is mediated through a Pertussis Toxin-sensitive G-protein. It was found that natriuretic peptides ANP and CNP were able to stimulate basal GC activity and reverse the NaCl inhibitory effect. These results indicate that both GC-A (ANP sensitive) and GC-B (CNP sensitive) are present in the bovine trachealis and that an inhibitory G-protein regulates their functioning. The wasp venom derived peptide mastoparan was also shown to enchance the ANP and CNP effect on GC activity, but was not able to reverse the NaCl inhibitory effect. These data is indicative of the presence of a stimulatory G-protein in bovine airways through which mastopara exerts its effect on GC activity.

Palabras clave : Guanylyl cyclase-G protein- natriuretic peptides.