Resumo

GUERRA DE GONZALEZ, L et al. La Atropina actúa como un Inhibidor de las Fosfodiesterasas de los Nucleótidos Cíclicos del Músculo Liso Traqueal de Bovino. RFM [online]. 2002, vol.25, n.1, pp.87-89. ISSN 0798-0469.

Muscarinic antagonists (Atropine) increased the basal levels of cyclic nucleotides (cAMP and cGMP) in tracheal smooth muscle being this effect similar to the ones produced by Isobutyl methyl xantine (IBMX), which is a non-selective phosphodiesterase (PDE) inhibitor. In the work, we evaluated the effects of specific PDEs inhibitors, such as Rolipram^® (PDE IV) and Zaprinast^® (PDE V) in the presence of 10^-5 M atropine on the basal levels of cAMP and cGMP in bovine tracheal smooth muscle. Strips samples were obtained of different times of incubation in the presence of drugs and TCA soluble extracts ware prepared. They last material was employed to determine the cAMP and cGMP concentrations using radioinmunoassays. Our results showed that atropine synergistically increased the effects of PDE IV and V inhibitors. The data suggest that muscarinic antagonists such as atropine might activate a signal transducing cascade, which is associated to a PDEs inhibition in airway smooth muscle.

Palavras-chave : Cyclic Nucleotides; Muscarinic antagonist; Phosphodiesterases.