Resumo

MONTES, C; MIJARES, G  e  ALFONZO, M. La V-Atpasa de la Membrana Plasmática del Músculo Liso Traqueobronquial de Bovino, es estimulada por Metabolitos Glicolíticos. RFM [online]. 2002, vol.25, n.1, pp.90-92. ISSN 0798-0469.

The effects of different glycolytic intermediates were evaluated for V-ATPase activity associated with a plasma membrane fraction isolated from tracheal smooth muscle. Plasma membranes were prepared and extracted with KCl following a technique described in our laboratory. V-ATPase activity was assayed by quantities of Pi released as previously described. In the absence of KCl, the following compounds were assayed: 3-phosphoglycerate (3-PG), 2-phosphoglycerate (2-PG), 3-phosphoglyceraldehyde, glycerol-3-phosphate and dihidroxyacetone phosphate. Our results showed that a phosphate group in 3-position of triose carbon structure is crucial for V-ATPase stimulation. In this sense, (0.1 mM 3-PG) showed the maximal activation being sensitive to Bafilomycin A₁ indicating the V-ATPase involvement.

Palavras-chave : V-ATPase; Glycolytic intermediates; Plasma membranes; Smooth muscle.