Abstract

ALVAREZ, Marco et al. El producto de excreción de shigella dysenteriae induce cariólisis y calcificación en el tejido hepático de embrión de pollo. "ex vivo" e "in vitro". RFM [online]. 2006, vol.29, n.1, pp.16-22. ISSN 0798-0469.

Objective: The aim of this study was to characterize, histologicaly and morphometricaly the chick embryo hepatic tissue alteration caused by the action of excretion products of Shigella dysenteriae "ex vivo" and "in vitro". Methods: Hepatic tissue of 8 day-old chick embrion development, "ex vivo", and hepatic cell culture "in vitro" in Hanging-drop techniques were used. Both systems were embedded in the excretion products of Shigella dysenteriae obtained from the supernatant of Shigella dysenteriae Type I. Three controls were defined: a) Tyrode solution, b) brain-heart broth infusion (CCC), c) supernatant of E. coli 0157:H7, not toxigenic. After 1 h of treatment, the tissue and cells were fixed in formol-zinc. Histological findings were obtained with hematoxylin eosin and Von Kossá staining. In order to carry out the morphometricaly examination, an imagen analysis in microscopic morphology techniques with ImagenJ 1.36 version was applied. Results: The embryonic hepatic tissue treated, "ex vivo", with excretion products of Shigella dysenteriae show: a loss in the arrangement of the endothelial cells, sinusoidal space was more open, nucleus with loss of the nuclear content and presence of calcium phosphates on the endothelial cells that covered the central vein, with regard to controls groups. The hepatic cells treated "in vitro" showed: narrow forms, abundance of lipid vesicles of great size, significantly reduction of pixels per nuclear area on grey scale between 85-168 and increase of the white region on grey scale between 169-255, when compared to control groups. Conclusions: The excretion products of Shigella dysenteriae induced karyolysis and calcified center formation. Both phenomena probably defined signs of excretion product of Shigella dysenteriae injury on embryonic hepatic tissue.

Keywords : Excretion; Ehigella dysenteriae; Hepatic tissue; Karyolysis; Calcification; Chick embryo.