Abstract

GALLARDO, Alfredo; MARQUEZ, Alejandro; PASTORE, Georget  and  FERNANDEZ, Luisa Elena. Experiencia en intercomparación de parámetros hematológicos empleando sangre fresca. RFM [online]. 2007, vol.30, n.1, pp.50-54. ISSN 0798-0469.

A dynamics of experimental external evaluation in hematology was carried out, employing fresh blood without preservatives. It was corroborated its homogeneity and biosafety, it was transported in chain of cold to the 33 participating clinical laboratories and established the reference values in our laboratory. The coefficient of variation found were: 5.1 % Hemoglobin (HB), 5.4 % haematocrit (HTO), 8.7 % white blood cells (WBC), 4.2 % red blood cells (RBC), 8.0 % platelets (PLT), 3.2 % MCV, 4.2 % MCHC and 4,3 % MHC, all considered satisfactory. The deviation index (DI) obtained were: satisfactory (DI<2.0) 97.0 % WBC, 90.9 % HB, 66.7 % PLT, 63.6 % RBC, 48.5 % HTO, 27.2 % MCV; unsatisfactory (DI>2.0) 81.8 % MCHC and 93.9 % MHC, this probably related to calibration problems of the instruments. Fresh blood constitutes a practical and economic alternative that can impulse the realization of intercomparison programs at local level.

Keywords : Intercomparison; External evaluation; Hematology tests; Fresh blood sample; Quality control.