Resumen

TORO, Elsa S; O MIRO, Astrid; UGARTE, Carmen I  y  LARREA, Francisco J. Pulse Field Gel Electrophoresis in molecular typification of strains isolated from a Salmonellosis outbreak caused by consumption of Salmonella Javiana contaminated cheese. INHRR [online]. 2010, vol.41, n.2, pp.22-26. ISSN 0798-0477.

The aim of this work was confirmation through Pulse Field Gel Electrophoresis (PFGE) that Salmonella isolated from the food implicated in the outbreak (white cheese) was responsible for the event. The white cheese sample showed a high count of Coli orms,f E. coli and S. aureus. Moreover, Salmonella was pre ent,s which indicated inadequate sanitary conditions and possible fecal contamination. The suspected Salmonella strains isolated from patients and the food sample, were confirmed using conventional biochemical techniques, serotyping according to the White-kauffmann-Le Minor scheme and antibiotics sensibility (Am picillin, Trimetroprim-Sulfametoxazole, Ciprofloxacin, Amoxicillin, Clavulanic Acid, Cloranfenicol, Ceftriaxone and tetraciclin) following kirby-Bauer’s technique. The Salmonella strains were identified as Salmonella Javiana [1,9,12:I, z₂₈:1,5]and were sensitive to all the antibiotics tested. The molecular typing of the strains was performed using PFGE, according to the PulseNet standardized protocol for Salmonella. The pattern analysis was studied using Bionunella merics program, version 4.0, which showed that the Sal monella Javiana isolates from patients as the food sample had an identical restriction pattern in size and fragments number. The incidence of a unique pattern of PFGE (similarity coefficient 100%) between isolates demonstrated that the cheese contaminated with Salmonella Javiana was responsible for the family outbreak.

Palabras clave : Salmonella Javiana; tipificación molecular; electroforesis en campo eléctrico pulsado; epidemiología molecular; enfermedad transmitida por alimentos; Salmonelosis.