Abstract

ALEGNA, Rada R; ROSCHMAN-GONZALEZ, Antonio; STRAUSS R, Mirian  and  TEJERO, Felix. Expresión de HSP60 en infecciones murinas experimentales por Trypanosoma evansiHSP60 expression in experimental murine infections by Trypanosoma evansi. INHRR [online]. 2011, vol.42, n.1, pp.52-57. ISSN 0798-0477.

Heat shock protein induction has been related to organism defense in diverse kinds of infection. This investigation examines Hsp60 accumulation in Mus musculus infected ex - perimentally with Trypanosoma evansi isolated from horse. This parasite is the causal agent of the “derrengadera” in Venezuelan planes. Normal and immunosupressed (cyclophosphamide( 250 mg/kg).) NMRI mice (♂; 20 gr body weight) were intra-dermal inoculated with 1 trypomastigote/ gr body weight; control groups were injected with distillated water in the same conditions. Hsp60 accumulation was analyzed by Western blot with a monoclonal antibody in plasma samples obtained each four days post-inoculation. Parasitemia levels were also registered. We observed a differential accumulation of Hsp60, which correlates with pa - rasitemia and immune conditions of the host. These results suggest an immunological control of Hsp60 expression that depends on the physiological condition of the host and parasitemia.

Keywords : Trypanosoma evansi; Hsp60; cyclophospha - mide.