Abstract

FARIAS, Zulmary et al. Comparative study of culture and sensitivity polymerase chain reaction in the diagnosis of Bordetella pertussis from nasopharyngeal swab specimens. INHRR [online]. 2015, vol.46, n.1-2, pp.38-50. ISSN 0798-0477.

Whooping cough is an infectious disease caused by Bordetella pertussis, diagnosed by culture as a reference technique, which has low sensitivity as limiting. It is because of this that the present work focused on evaluating the PCR technique endpoint amplifying IS481 and PT sequences, as an alternative methodology diagnostic from 100 paired samples of nasopharyngeal swabs from patients from different regions of Venezuela. Such paired samples comprised a swab for bacterial culture and the other for detection of specific DNA. Microbiological identification of B. pertussis included the isolation of the microorganism in agar Regan-Lowe, biochemical identification and confirmation by Coagglutination. The analysis of the results was performed using the SPSS program 21.0.0., as a statistical tool using the McNemar test. The sensitivity obtained by the PCR protocol endpoint was 50%, consistent with previous reports. According to the value of p = 0.002 obtained, detection of B. pertussis by the two methods showed a difference for the diagnosis of pertussis statistically significant, so it is not indifferent both diagnostic techniques employed. However, it is recommended to use both methods in combination to increase the likelihood of diagnosis.

Keywords : Bordetella pertussis; microbiological culture; PCR; diagnostic sensitivity; whooping cough; IS481 gene; PT gene.

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