Abstract

AGUILAR MEJIA, Marwan Said; ROJAS MENDOZA, Miguel Antonio; MICHELLI GAGO, Pedro Jose  and  CELAYA, Joseba. Evaluation of the expression of cellular markers p16 INK4a, PCNA and E-cadherin by immunohistochemical techniques in cervical lesions caused by human papilloma virus. INHRR [online]. 2015, vol.46, n.1-2, pp.51-67. ISSN 0798-0477.

Infections human papillomavirus (HPV) influence the expression of cell cycle proteins and produce changes in the cervical epithelium that promote the development of cervical intraepithelial neoplasia (CIN); HPV genotypes are grouped into HPV low risk or highoncogenic. Viral oncoproteins E6 and E7 of high-risk HPV (16 and 18), bind to various cell cycle regulators, therefore they are related to high-grade lesions and cervical cancer. The aim of this study was to evaluate 36 cervical biopsies by immunohistochemistry and measuring the expression of the following cellular markers: Nuclear Antigen Cell Proliferation (PCNA), the cell adhesion protein E-cadherin is inactivated in the initial moments of invasion and metastasis, p16 expression-INK4 protein is a cyclin inhibitor involved in the G1 checkpoint in the cell cycle cervical lesions. The results showed that in all samples tested increased the expression of p16INK4a (95.86%) and PCNA (92.27%) in lesion areas compared to areas without injury and a decrease in expression was obtained Ecadherin (18.94%) in areas with injury. In conclusion, the p16INK4a cell marker (P <0.0033) showed a better predictive performance of the evolution of lesions PCNA and Ecadherin.

Keywords : Human Papilloma Virus; Cervical lesions; Immunohistochemistry; sexually transmitted infections.

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