Revista Científica
versión impresa ISSN 0798-2259
Resumen
PEROZO, Francisco et al. Utilization of FTA® Cards for the Molecular Diagnostic of Newcastle Disease Virus in Allantoic Fluid Samples. Rev. Cient. (Maracaibo) [online]. 2006, vol.16, n.2, pp.118-123. ISSN 0798-2259.
The feasibility of using FTA® cards (Flinders Technology Associates) for inactivation and transportation of allantoic fluid infected with Newcastle disease virus (NDV) was evaluated. Serial dilutions of allantoic fluid with a titer of 108.8 ELD/50 of LaSota strain NDV were loaded on the FTA® cards and analyzed after 24 hour, 7 and 14 days using reverse transcriptase-polimerase chain reaction (RT-PCR). The lowest virus concentration that could be detected from the FTA® cards was 105.8 ELD/50. The detection of the inactivated virus was possible after 14 days of virus inactivation. No virus re-isolation in embryonating eggs was possible from the cards. No negative effects of the FTA® card inactivation on the nucleotide sequences were observed, allowing the determination of its virulence by direct nucleotide sequencing of the F protein cleavage site, resulting in a lentogénic strain in concordance with the initial virus applied on the cards. It can be conclude that FTA® cards are a valid alternative for NDV sampling, inactivation and molecular diagnostic with a high degree of biosecurity.
Palabras clave : Newcastle disease virus; FTA® cards; inactivation; molecular detection.