Resumo

MEJIA RODRIGUEZ, José Andrés et al. Obtention of Lactobacillus strains. : In-Vitro Characterization as Potentials Probiotics.. Rev. Cient. (Maracaibo) [online]. 2007, vol.17, n.2, pp.178-185. ISSN 0798-2259.

In this work three hundred and sixty strains of microorganisms were isolated from feces of nursing children and vaginal samples of ten women. Only twenty-two of the strains were identified as Lactobacillus. They were resistant to hostile conditions such as pH 3 and 0.30% of bile salts. It was detected antimicrobial activity in the growth supernatant against: Bacillus subtilis ATCC 60519, Candida albicans ATCC 14053, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 and against enteropatogenics bacteria strains such as Shigella sonnei, Escherichia coli poli I and Salmonella typhi isolated of infants with diarrhea. It was observed resistance of Lactobacillus strains to a mixture of 1.25 µg of trimethoprim and 23.75 µg of sulphamethoxazole. However they were sensitive to 10 µg of ampiciline. These strains can be considered as posibles probiotics of great utility in the dairy industry.

Palavras-chave : Acid lactic bacteria; Lactobacillus; probiotics.