Resumo

GATICA P., Cristina  e  GESCHE R., Erika. "The Five Plate Method" for the Detection of Antibacterial Residues in Milk.. Rev. Cient. (Maracaibo) [online]. 2007, vol.17, n.3, pp.231-238. ISSN 0798-2259.

Serious problem come out for public health as for the milk industry when antibiotics residues appear in milk products. It has become necessary to have a standarized method, of low cost, easy to perform and able to detect most of the antibiotics used now a days. The aim of this work was the evaluation of the sensibility of a novel microbiological technique able to detect these residues in milk, in order to go with the evolution of the use of these drugs in dairy cows in Chile. The technique proposed was named "The 5 Plates Method" and uses as sensible strains Bacillus stearothermophilus at pH 7.0, Bacillus subtilis at pH 6.0, 7.2 y 8.0 y Escherichia coli at pH 8.0. Eighteen antimicrobials where studied, those of greater offer in Chile for dairy cows (ampicillin, cefoperazone, ceftiofur, cloxacillin, penicillin G, pirlimycin, nafcillin, dihydrostreptomycin, gentamicin, neomycin, enrofloxacin, spiramycin, chloramphenicol, lincomycin, oxitetracycline, tetracycline, sulfadimidine and suldadoxin). The most sensitive plate was determined for each one of them. Each antimicrobials analysed with "The 5 Plates Method" was detected in one of the 5 plates. Later, on the plate determined as the most sensitive to each antimicrobial, calibration curves where made to point out the minimum detectable concentration for each one of them, resulting to be each plate sensitive to at least 2 or more antimicrobials demonstrating therefore that this method has an suitable sensitivity to these antimicrobial.

Palavras-chave : Residues; antimicrobials; milk; microbiological method.