Resumen

NUNEZ OCHOA, Luís; TRIGO TAVERA, Francisco José; MADRID MARINA, Vicente  y  GUTIERREZ LOPEZ, Andrés. Distribution of b-galactosidase by Adenoviral Transfer as a Model for Intralymphonodal Gene Therapy in Dogs With Spontaneous Multicentric Lymphosarcoma.. Rev. Cient. (Maracaibo) [online]. 2007, vol.17, n.5, pp.464-472. ISSN 0798-2259.

Gene therapy administration in cancer is mainly performed by intravenous, oral, and in situ routes, with viral or nonviral delivery vector systems. Systemic administration frequently transfers the therapeutic gene to neoplastic tissue and as well as to other organs. The objective of this study was to evaluate the distribution expression of Lac Z reporter gene by adenoviral transfer administered by intralymphonodal route (ILNR) in dogs with lymphosarcoma. The distribution of b-galactosidase protein expression by a non replicative recombinant adenovirus (Adb-gal) delivery was determined in six dogs with spontaneous multicentric lymphosarcoma, 72 h after ILNR administration using X-gal chromogenic substrate. The doses administered were 0 (control), 1.35 X 10¹⁰, 2.53 X 10¹⁰, 6.10 X 10¹⁰, 18.38 X 10¹⁰ and 153.85 X 10¹⁰ viral particles (VP) /kg. The expression was manifested in 100% of lymphocytic tissue, including lymph nodes and spleen. The infiltrated organs with neoplastic lymphocytes: liver, bone marrow and lungs were positive but with lower intensity. Conclusion. The expression of b-gal was restricted to neoplastic lymphocytic tissue and metastatic sites. The ILNR would enhance gene therapy efficacy to a specific cell type and permit the delivery of lower doses, which result in reduced toxicity and may also potentially be less immunogenic. This suggests that adenoviral gene therapy by ILNR is a potential model of administration in animals and human beings with lymphosarcoma and also for metastasis to lymph nodes.

Palabras clave : Gene therapy; lymphosarcoma; adenovirus; intralymphonodal route; dog.