Resumen

MORALES LOREDO, Alberto et al. Correlation Between PCR and Tuberculin Test for Detection of Mycobacterium tuberculosis Complex Organisms in Bovine Cattle.. Rev. Cient. (Maracaibo) [online]. 2008, vol.18, n.1, pp.17-21. ISSN 0798-2259.

In Mexico, the bovine tuberculosis (TB) is associated with both regionalization of the country, considering the levels of prevalence of the disease and restrictions in commercialization and/or exportation of cattle. In Mexico, the diagnosis of the bovine tuberculosis is conducted by using the tuberculin test in live animals, bacteriologic isolation and histopathological analysis in postmortem. Although the low sensibility of tuberculin test, is used to sent the animal reactors to slaughter and maybe the animal is not infected. Considering that some diagnosis methods are not sufficiently reliable and specific to detect the presence of the casual agent of this disease, the use of the polymerase chain reaction (PCR) test was evaluated from bovine nasal mucus. For the detection of the Mycobacterium tuberculosis complex, PCR of nasal mucus was better than Caudal-fold Tuberculin Test (CTT). The sensibility of PCR compared with CTT was 90.5% and specifity was 58.3%. The PCR of nasal mucus can use in buy and sell operations and cattle expositions to prevent the contagious and the introduction the pathogens in health cattle.

Palabras clave : Tuberculosis; Tuberculin; Mycobacterium; molecular diagnosis; PCR.