Abstract

BOEZ CONTRERAS, Francisco J; HERNANDEZ, Ludwing  and  VILLAMEDIANA MONREAL, Patricia C. Structural Study of Meiotic Spindle of Vitrified Bovine Oocytes.. Rev. Cient. (Maracaibo) [online]. 2008, vol.18, n.3, pp.253-261. ISSN 0798-2259.

Bovine oocytes vitrification has been used as one of the most promising cryopreservation protocols. Fear of the effects of cryoprotectant additives on the oocytes structural integrity have been posed. In this study, trials were conducted to evaluate the effects of vitrification over meiotic spindle structure and development capability in bovine oocytes. Based in this evidence, oocytes recently released from their follicles and in vitro matured were exposed at room temperature to vitrifying solutions (ethylenglicol and sucrose), also immature and in vitro matured oocytes were vitrified. Oocytes were stained in toto to assess nuclear maturation stage and meiotic spindle structural alterations were studied by immunocytochemistry. Oocytes at GV stage are more sensitive to the toxic effects caused by cryoprotectant agents at room temperature and to the cryopreservation procedure, assessed through meiotic progression and immunocytochemical analysis of meiotic spindle. The most frequently encountered anomaly was absence of meiotic spindle. Meiotic spindle structure in vitrified and IVM oocytes endure better the cryoinduced injuries compared with oocytes vitrified the GV stage. It is recommend the vitrification of in vitro matured bovine oocytes.

Keywords : Vitrification; oocytes; bovine; MIV; inmunocytochemical.