Resumen

MALDONADO, Jesús et al. The ring test and indirect elisa for brucella detection in milk on dual purpose bovine herds in the state of Lara, Venezuela. Rev. cient. (Maracaibo) [online]. 2010, vol.20, n.3, pp.240-244. ISSN 0798-2259.

The efficient control of the Bovine Brucellosis needs the opportune detection of the disease in the herds. Traditionally the Ring Test has served to realize epidemiological alertness of Brucellosis to level of dairy herds, nevertheless, this technology presents changeable behaviors depending on the number of animals that contribute milk sample and presence of milk of cows with mastitis or colostrum in the sample. In addition, the Ring Test is a subjective test, product of the visual appraisal of the analyst. As alternative method there have developed diverse kits of indirect ELISA (ELISAi) that allow the diagnosis of the Brucellosis in samples of milk. ELISAi’s formats show a great sensibility and results are measured by a spectrophotometer, which diminishes the human mistake of appraisal. In Venezuela most of the milk production is obtained from half-caste animals of dual purpose, generally raised under sanitary deficient conditions and by great variation in the number of animals that compose the herds, united to important rates of mastitis and a scanty control the period of withdrawal of the colostrum. A study was realized to know the status of the Brucellosis in the Edo. Lara - Venezuela, taking 510 samples of milk in 258 Units of Production, to fulfil Ring test and ELISAi, thinking that 27.25% was Not Negative to the Ring Test and 13.14 were positive to ELISAi. Both tests indicate that in the analyzed herds of the Lara State the disease exists. It was observed a relative sensibility of the Ring Test on having compared it with ELISAi of 56.72%, whereas the relative specificity reached 77.20%. This low sensibility carries in a high number of false negative samples, which is translated in faults of the epidemiological system of alertness.

Palabras clave : Brucellosis; ring test; indirect ELISA; relative sensitivity; relative specificity.