Abstract

COVA, Luis J et al. In vitro pathogenicity of Beauveria brongniartii (Sacc.) Petch against Musca domestica (L.) as a possible strategy for biological control in livestock areas. Zootecnia Trop. [online]. 2009, vol.27, n.2, pp.113-120. ISSN 0798-7269.

An experiment was carried out in order to study, in laboratory condition, the pathogenicity of Beauveria brongniartii (strain LF-05) against Musca domestica. The conidia of B. brongniartii were obtained from Perú. The fungus was propagated in potato-dextrose-agar culture medium, and later in semi-crude sterilized rice. F1 flies were obtained from wild specimens which were captured with mesh in a poultry breeding farm (Betijoque, Trujillo state, Venezuela). In the laboratory, one hundred and sixty adult flies from 3 days of age, divided into 8 lots with 17 to 40 per bottle flies were anesthetized with ether, and then applied B. brongniartii suspensions diluted in water from 108 conidia/mL, resulting in sis treatments: control, 1.2 x 103, 1.2 x 104, 1.2 x 105, 1.2 x 106, and 1.2 x 107 conidia/mL. Healthy controls with 100 flies were used with only ether as anesthesia. The counting of fallen flies, both in the experimental and bottles at checkpoints, it was up to 24 days. Using the Probit® methodology, it was found that the LT50 and LT95 were 11.08 and 13.25 days earlier than control group, respectively. Results showed that spores of B. brongniartii (strain LF-05), at 1.2 x107 conidia/mL, resulted in 95% mortality of M. domestica in 9.27 days. The use of nebulizations with similar concentration of spores to fly control in local production units of poultry and cattle were speculated.

Keywords : Beauveria brongniartii; Musca domestica; biological control; pathogenic action; entomopathogenic fungi.

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