Resumen

AHUJA AGUIRRE, Concepción; MONTIEL PALACIOS, Felipe; PEREZ HERNANDEZ, Ponciano  y  GALLEGOS SANCHEZ, Jaime. Alternative medium for in vitro production of bovine embryos. Zootecnia Trop. [online]. 2009, vol.27, n.3, pp.277-284. ISSN 0798-7269.

The objective of this study was to evaluate the blastocyst development rate for in vitro produced bovine embryos using either the conventional culture medium for bovine embryos or culture media used for the in vitro production (IVP) of human embryos. Oocytes were obtained from ovaries of slaughtered Zebu cows. Ovarian follicles were aspirated and the cumulus-oocytes complexes (COC) with at least two layers of intact cumulus cells were matured for 22 to 24 h. Following that, COC were fertilized with frozen-thawed bovine semen and coincubated for 20 to 24 h. The presumptive zygotes were cultured for 48 h in KSOM medium or modified HTF medium. On days 3 and 5, post-in vitro fertilization (IVF) embryos were placed into new drops of KSOM or IVC-Two/IVC-Three. For both culture types, the cleavage rate was determined on day 3 post-IVF and the embryo development rate to blastocyst stage was evaluated on days 7 to 9 post-IVF. In vitro maturation rate was 89.7%. Cleavage rate was 82.3% for oocytes cultured in KSOM and 80.1% for oocytes cultured in HTF (P>0.05). Blastocyst development rate was 31.6% for embryos cultured in KSOM and 29.5% for embryos cultured in HTF/IVC-Two/IVC-Three (P>0.05). In conclusion, in vitro blastocyst development rates were similar for both the embryos cultured in the conventional medium for IVP of bovine embryos and the embryos cultured in media used for the IVP of human embryos, thus, both types of media can be used for the IVP of bovine blastocysts with acceptable results.

Palabras clave : Blastocyst; bovine; embryo; in vitro production.

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