Resumen

PINEDA, Juan et al. In vitro fungistatic property of propolis on three Colletotrichum gloeosporioides isolates. Zootecnia Trop. [online]. 2010, vol.28, n.1, pp.83-91. ISSN 0798-7269.

Antracnose caused by the fungus Colletotrichum gloeosporioides, is a pathology that causing fruit rot and reducing the commercial value of many fruits in Venezuela. In order to evaluate the antifungic property of Apis mellifera propolis on isolates of C. gloeosporioides that affect fruits of avocado (Persea americana), papaya (Carica papaya) and passion fruit (Passiflora edulis), a study was done using a propolis collected from the Guaremal Apicultural Station, UCLA. The treatments were: control (no treated) and dilutions of the propolis in ethanol to 0%, 15%, 20% and 30%. On PDA culture medium, was placing in each Petri dish four circles of paper filter, previously soaked during 5 minutes in the propolis to the necessary dilution, and a disc of the respective strain of C. gloeosporioides in the center of the same one. The measurements were made daily until the control treatment reached the maximum ratio. In the propolis dilution the presence of secondary metabolites was determined. Significant differences between treatments were observed as far as development of the fungus, when comparing them with control treatment, the smaller development of the fungus happened, for the stocks of avocado and passion fruit, with propolis to 30% (27.6 mm and 28.9 mm, respectively), and for the one of papaya, with propolis to 20% (25.3 mm). The inhibition of the growth was around 30%. The propolis dilutions at 15, 20 and 30% statistically did not present significant differences to each other, thus those were similar in their effect. A suppressor effect of the propolis on the micelial growth of C. gloeosporioides and presence of flavonoids compounds in extract were demonstrated

Palabras clave : antracnose; Colletotrichum gloeosporioides; honeybee propolis; fungistatic.

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