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Zootecnia Tropical

versión impresa ISSN 0798-7269

Resumen

MALDONADO, Jesús et al. Comparative study of rose bengal and competitive elisa tests for brucellosis in dual purpose herds in Lara State, Venezuela. Zootecnia Trop. [online]. 2012, vol.30, n.2, pp.135-145. ISSN 0798-7269.

Brucellosis is a zoonotic disease that negatively affects milk production in Latin America. Infections with Brucella abortus bacteria observed in human populations are fundamentally caused by ingestion of non-pasteurized milk by-products. In Venezuela, most of the milk production is obtained from crossbred dual purpose herds, which are generally under defi cient sanitary conditions. A signifi cant amount of milk produced is used for production of non-pasteurized cheese for human consumption. In Venezuela, the offi cial screening test for brucellosis is Rose Bengal test (RBT), leaving competitive ELISA test (cELISA) to only defi nitively confi rm results obtained from RBT. A trial was performed to assess the status of brucellosis in Lara state, Venezuela. Initially, milk ring tests were performed in 276 farms, with 30% of them testing positive. Serum samples were collected from all animals of farms that tested positive to ring test. A total of 13.351 serum samples were collected and tested with both RBT and cELISA assays. Positive index observed with RBT was lower when compared to cELISA (0.51% vs 7.33%, respectively). Furthermore, both tests confi rm that positive index in Lara state is very low. However, relative sensitivity observed in RBT is very low when compared to cELISA (6.44%). This low sensitivity could result in a high number of false negative animals that, consequently, will not be eliminated from the herds. Thus, this would constitute a constant source of infection to other animals and people

Palabras clave : Brucellosis; Zoonotic disease; Rose Bengal Test; competitive ELISA; positive index; relative sensitivity.

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