Resumo

CENTENO BRICENO, Sara  e  CARRERA JASPE, Yohanna. ANTIFUNGAL AND ANTIAFLATOXIGENIC ACTIVITY OF EXTRACTS OF Melissa officinalis (LAMIACEAE) ON Aspergillus flavus. Saber [online]. 2013, vol.25, n.2, pp.185-192. ISSN 1315-0162.

Because  the  use  of  fungicides  in  agricultural  crops  has  led  to  the  development  of  fungal  resistance, environmental pollution and public health hazards, the antifungal activity of the extracts from wild plants has been studied, seekingnew alternatives in the control of fungi and mycotoxins. Therefore, the objective of this research was to evaluate the antifungal and antiaflatoxigenic activity of extracts of Lemon balm (Melissa officinalis) on Aspergillus  flavus.  Extracts  of  Melissa  officinalis  were  obtained  using  ethanol  80.00%,  and  after  the  alcohol evaporated, aqueous solutions were prepared for testing, and from them it was determined: (a) the antifungal activity of the extracts, (b) minimum inhibitory concentration (MIC) of the extracts on Aspergillus flavus  and (c)  antiaflatoxigenic  activity  using  the  method  of  competitive  enzyme  immunoassay  (ELISA).  The  results  of the antifungal activity showed that the extracts produced averaged halos of inhibition of 22 mm on the fungus tested.  It  also  presented  a  minimum  inhibitory  concentration  of  23.30  mg/mL.  The  extract  reduced  aflatoxin concentrations  of  5,  15  and  45  mg/kg  in  a  90.60%,  85.33%  and  84.71%,  respectively.  In  conclusion,  Melissa officinalis  extracts showed antifungal and antiaflatoxigenic activity on Aspergillus flavus , largely inhibiting its growth and significantly reducing aflatoxin concentrations.

Palavras-chave : Natural extracts; contaminants fungi; aflatoxins.

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