Resumo

LEMUS-ESPINOZA, María T; MANISCALCHI, Badaoui  e  LEDEZMA, Eliades. Morphometric alterations in cells from exposed Microsporum canis to different ajoene concentrations Druvic. Saber [online]. 2013, vol.25, n.3, pp.279-284. ISSN 1315-0162.

From three strains of Microsporum canis isolated from patients with Tinea capitis, macroconidia were extracted. These were exposed during 13 cosecutive days to growing concentrations of ajoene, between 1-300μM, using the microdilution method in plate M38-A2. The structures were monitored morphometrically to observe the effect of this compound on the fungus. The comparison between the normal conidia and those exposed to the compound showed morphologic diferences. The in vitro effects were time dependent, able to induce drastic and irreversible morphologic changes in the fragmoconidia, evidenced in the noticeable increase of thickness of cytoplasmic membrane and in the loss of proportion of the interceptal spaces of conidia. Ajoene, as antimicotic agent, inhibited conidial growth, which is evidenced under the microscope by the deformation of the normal architecture of cells. The loss of function and viability of conidia, which became irregular, is probably associated to alteration of the synthesis of plasmic and interconidial membrane.

Palavras-chave : Morphometric analysis; microdilution; Tinea capitis.

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