Resumo

FLORES FERNANDEZ, Evelin Margarita; CARABALLO SUNIAGA, Susanny del Carmen  e  ALBARADO YSASIS, Luzmila Sofía. Staining characteristics of Neisseria gonorrhoeae by modified differential fluorescent staining method. Saber [online]. 2014, vol.26, n.3, pp.281-288. ISSN 1315-0162.

There are few fluorescent staining techniques used in studies of the bacterial cell cycle, so that the staining characteristics of Neisseria gonorrhoeae were analyzed by the modified differential fluorescent staining method and the association with the presence of DNA and RNA. The purpose of this study was to provide evidence on the reaction mechanism of modified differential fluorescent staining method, as well as offering the technique as a complementary tool in studies of the cell cycle. The cell cycle was evaluated in isolates of N. gonorrhoeae transferring a culture in optimal growth to BHI broth with 5% human blood. Aliquots were taken to prepare slide spreads for staining, before and after treatment with DNase and RNase at 5 to 120 min intervals, and the McNemar test was applied to verify the association of the staining reactivity before and after enzyme treatment. In the cell cycle, the bacteria staining was predominantly slightly fluorescent orange and the color sequence in the cycle was yellow, orange and green. Cells which stained orange or yellow, after the enzyme treatment, changed to slightly fluorescent green or almost imperceptible green, with statistically significant association (p < 0,05) between the yellow and orange slightly fluorescent or fluorescent with the presence of DNA and RNA. We conclude that DNA and RNA are involved in the yellow and orange dye by modified differential fluorescent staining method in the cell cycle of N. gonorrhoeae.

Palavras-chave : Cell cycle; DNA; RNA; DNase; RNase.

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