Abstract

PORTELES, Maribel; RODRIGUEZ, Dorian; ULACIO, Dilcia  and  TORRES, Jhonathan. Determination of races and groups of vegetative compatibility of Fusarium oxysporum f. sp. cubense in Musa in the states of Carabobo, Cojedes, Guarico, and Miranda, Venezuela. Saber [online]. 2015, vol.27, n.2, pp.334-340. ISSN 1315-0162.

There are various problems affecting banana production, however, Panamá disease, caused by Fusarium oxysporum f. sp. cubense (Foc), is considered the most devastating disease worldwide. In Venezuela, the disease has been reported in ten of the twenty three states; in this research, the presence of the fungus was studied in four states not studied before (Guárico, Cojedes, Carabobo y Miranda). Banana plantlets were generated from 'Apple', 'Bluggoe' and 'Grand Nain' cultivars, in order to identify the races of Foc. Using a non probabilistic sampling, 12 farms were surveyed and symptomatic plants with Panama disease were located, selecting 2-3 plants per farm which were sampled by puncturing the pseudostem. Fifty isolates were obtained. For pathogenicity test, inoculated rice with Foc was used at a concentration of 6 x 10⁵ conidia/mL, and was maintained at environmental temperature for 8 days to grow. Afterwards, it was added to the substrate, the plantlets were planted and incubated for up to 2 months. A complete random design with a factorial arrangement was used. At 1-4 weeks, disease symptoms were observed on ‘Bluggoe’ and ‘Apple’ cultivars, concluding that in the areas studied the disease is caused by races 1 and 2 of Foc. Simultaneously, studies were performed to determine the vegetative compatibility group (VCG), for which nit mutants were generated, identified and challenged with GCV testers. Isolates were incompatible with the standards used.

Keywords : GCV; nit mutant; Panamá disease.

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