Resumo

VILLALOBOS, Luz Bettina; CAVAZZA, María Eugenia  e  ORTIZ-PRINCZ, Diana. Detection of cagA gene and typing vacA gene of Helicobacter pylori in biopsies of patients with gastric symptoms in Cumana, Sucre state, Venezuela. Saber [online]. 2015, vol.27, n.3, pp.430-440. ISSN 2343-6468.

Helicobacter pylori has been associated with gastric diseases such as gastritis, ulcers, lymphoma gastroduodenal and is considered a risk factor for the development of gastric cancer. The aim of this study was to characterize vacA genotypes and to determine the presence of cagA gene in biopsies from patients with gastric symptoms treated at the University Hospital Antonio Patricio de Alcalá of Cumaná, Venezuela. Sixty nine patients with endoscopic indication were evaluated using the polymerase chain reaction (PCR). Only 42 (60.86%) of those patients were positive for amplification of vacA and/or cagA and of these, 54.76% amplified for both genes. The predominant genotype was m1s1 (61.90%) followed by mixed infections (33.33%), being the most frequent associations among strains with genotypes m1s2, m2s2 and m1s1. From the biopsies performed 72.5% were negative for the cagA gene. However, this was found to be associated mainly to genotype m1s1 (17.39%). Statistically highly significant correlations were found among the allelic forms m1 and s1 (ρ = 0.732), s2 and m2 (ρ = 0.643), significant m1 and s2 (ρ = 0.279). Gene cagA showed highly significant correlations with m1 (ρ = 0.509), m2 (ρ = 0.447) and significant with s1 (ρ = 0.319) and s2 (ρ = 0.263). These results show the existence of co-infections with different genotypes of H. pylori in the studied population. They, would explain in part the low incidence of severe gastric pathologies despite being present in the region virulent genotypes of this bacterium. The high percentage of coinfections observed could be the result of the influence of geographic and ethnicity factors in the region where the study was conducted.

Palavras-chave : H. pylori; genotyping; gastritis.

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