Resumen

GUEVARA, Miguel et al. Influence of salinity and irradiance on growth and biochemical composition of a new strain of Dunaliella salina from the Araya salt works, Venezuela. Saber [online]. 2016, vol.28, n.3, pp.494-501. ISSN 2343-6468.

Dunaliella salina is a microalga used for the production of metabolites of high industrial and pharmaceutical value. The combined effect of irradiance and salinity on growth, pigment production and biochemical composition of this strain were evaluated in order to contribute to the knowledge of the physiological responses of this new isolate of D. salina from Araya saltworks, to changes in the conditions of its environment. It was aslo intendend to increase the variety of native microalgae species which can serve as sources of bioproduction for bioactive compounds, biomass and metabolites of application in pharmaceuticals and biomedical industries. Batch cultures of the strain of D. salina were conducted for 22 d at f/2 medium Guillard (0.88 mmol.L^-1 of nitrogen) at 27 ± 2°C, pH 7.8; photoperiod 12 h light: 12 h darkness, continuous aeration (150 mL.L^-1) in two salinities (40 and 250 UPS) and irradiances (195 and 295 μmolphotons.m^-2.s^-1). Population growth was evaluated daily and at the end of the trial the contents of biomass, pigments, proteins, lipids, carbohydrates and cell size were determined. The growth rate of the new strain of D. salina decreased with increasing salinity and irradiance. However, cell size, biomass, content of proteins, lipids, carbohydrates, total carotenoids and β-carotene increased with increasing irradiance and salinity. In addition, the potential of this strain for the production of β-carotene was shown.

Palabras clave : Microalgae; pigments; hipersaline; biotechnology.

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