Resumo

AREVALO, Z et al. Implementation of a protocol for the identification of Vibrio cholerae. Rev. Soc. Ven. Microbiol. [online]. 2002, vol.22, n.2, pp.128-130. ISSN 1315-2556.

Abstract Vibrio cholerae produces a powerful toxin coded by two genes: the operon ctxAB. Epidemiology studies have been limited by phenotypic techniques; from this it is necessary to establish a protocol for detection of the gene of subunity A (ctxA) that codes Vibrio cholerae toxin for which it was done genetic characterizations of 24 isolates of the Vibrionaceae family gotten from isolates of Bacteriology Laboratory of Sanidad Animal- CENIAP-INIA, from lisas, tilapias and cachamas. It was followed the methodology recommended by Fields et al. (1992) with modifications; this are the sequences of these primers 5'-CGGGCAGATTCTAGAC CTCCTG-3' y 5'-CGATGATCTTG GAGCATTCCCAC-3'. The not-O1 Vibrio cholerae isolates studied do not have the choleric toxin. Investigations about choleric toxin on environment isolates is necessary since isolates studied were gotten from físhes for human consumption which constitutes a public health risk

Palavras-chave : Protocolo; toxina; Vibrio cholerae.