Resumo

UZCATEGUI-NEGRON, M; SERRANO, JA; DIAZ-CORRALES, F  e  AVELLA, J.. Selective isolation of nocardia brasiliensis from soil samples from san Juan de Lagunillas, Mérida state, Venezuela, using a tetracycline medium. Rev. Soc. Ven. Microbiol. [online]. 2003, vol.23, n.1, pp.38-41. ISSN 1315-2556.

    20 soil samples collected from San Juan de Lagunillas, Estado Mérida (Venezuela) were studied. An agar tripticasa soy medium was used supplemented with tetracycline (5µg/ml) and cicloheximida (10µg/ml) for the isolation of the microorganisms that were present in the soil samples. 13 (65%) of the isolated strains were identified as nocardias. Later, these strains were identified through microbiological tests. From the total number of isolated Nocardia strains, the following proportion was observed: 10% of the isolated were identified as Nocardia asteroides, another 10% as Nocardia sp, and the remaining 45% were identified as Nocardia brasiliensis.     Tetracycline was used in the selective medium of isolation. This process allowed a greater isolation of Nocardia brasiliensis and to a lesser degree Nocardia asteroides.

Palavras-chave : Nocardia brasiliensis; muestras de suelo; Nocardia asteroides.