Resumo

CORREA DE ADJOUNIAN, MF et al. Mycobacteria in autopsy samples. Rev. Soc. Ven. Microbiol. [online]. 2004, vol.24, n.1-2, pp.8-11. ISSN 1315-2556.

Eighty three samples coming from autopsies (34 of lung tissue, 25 of ganglion tissue and 24 of leptomeninges) corresponding to 34 deceased patients with clinic suspicious of tuberculosis and/or HIV, were bacteriologically studied. In 10,8% (9/83) were mycobacterial growth: four M. tuberculosis and five non tuberculous mycobacteria (NTM): one (1) M. gordonae, one (1) M. vaccae and three that could not be identified biochemically. Six of these isolated were studied by PCR amplification of the IS6110 sequence. Three isolates (50%) were reconfirmed as mycobacteria belonging to the tuberculous complex (MTC). In the three remaining isolates, corresponding to NTM, IS6110 amplification was not obtained. However using amplification followed by polymorphism restriction analysis (PRA) of hsp65 gen segment, they were identified as M. porcinum, M. vaccae and M. gordonae type II.

Palavras-chave : Autopsy; mycobacteria; tuberculosis; non-tuberculous mycobacteria.