Resumen

ARRAIZ RODRIGUEZ, Nailet et al. Detection of Chlamydia trachomatis in endocervical swabs samples by direct immunofluorescence and polymerase chain reaction. Rev. Soc. Ven. Microbiol. [online]. 2006, vol.26, n.1, pp.14-18. ISSN 1315-2556.

Chlamydia trachomatis is a pathogen of high prevalence in urogenital infections; according to data of the WHO, annually 89 millions of new cases are detected at world wide level. With the purpose of comparing the method sensibility of direct immunofluorescence (DIF) and amplification techniques by PCR to the Chlamydia trachomatis detection in clinical samples, and to investigate the Chlamydia trachomatis prevalence in symptomatic and asymptomatic women; 54 endocervical swabbing specimens of patient that solicited gynecological attention in a Ambulatory of Maracaibo were analyzed. To investigate Chlamydia trachomatis by DIF Chlamydia Direct IF kit (bioMerieux^®) was used. The molecular detection was carried out by polymerase chain reaction (PCR-CTP), using the CTP1 and CTP2 oligonucleotide directed to sequences of endogenous plasmid of Chlamydia trachomatis that generated a 201 pb product. 5/54 samples were positive with PCR-CTP; while by DIF 3 cases was detected. 49 samples were negative for both tests The prevalence of Chlamydia trachomatis was of 5,55% for DIF and 9,26% for PCR-CTP Compared with PCR-CTP test, the sensibility of the DIF analysis was 60,00%. The molecular methods offer a diagnostic strategy that can contribute to the design of epidemiological control programs, directed to diminish the prevalence of this microorganism.

Palabras clave : Chlamydia trachomatis; DIF; PCR-CTP; molecular detection; urogenital infection.