Revista de la Sociedad Venezolana de Microbiología
versão impressa ISSN 1315-2556
Resumo
LEMUS ESPINOZA, Druvic; MANISCALCHI BADAOUI, María Teresa; HASSOUN, Mazn e VIZCAYA, Heycarid. Oxacyllin resistant Staphylococcus in white cheese manufactured at the Anzoategui State, Venezuela. Rev. Soc. Ven. Microbiol. [online]. 2008, vol.28, n.1, pp.48-54. ISSN 1315-2556.
The purpose of this study was to detect oxacyllin resistant Staphylococcus species in hard and soft white cheese manufactured by artisans at various municipalities of the northern area of Anzoátegui State. The Staphylococcus strains were isolated and identified using conventional methods and coagulase negative species were confirmed through API 32 Staph (BioMérieux) galleries; susceptibility to oxacyllin and other antibiotics was determined by the disk diffusion method and presence of the mecA gene with the MRSA Slidex (BioMérieux) latex test that identifies PBP2a protein. From 130 cheeses evaluated, we isolated 171 Staphylococcus strains, 26 of which (15,2%) were oxacyllin-resistant (61,5% with presence of the gene), distributed as follows: 14, S. epidermidis strains (8,2%); 6, S. capitis (3,4%); 2, S. aureus (1,2%); 2, S. hominis (1,2%); 1, S. warneri (0.6%); and 1, S. saprophyticus (0.6%). Presence of the protein PBP2a codifying gene was detected in both homoresistant (76.9%) and heteroresistant (23.1%) strains. S. haemolitycus was 100% oxacyllin sensitive. Presence of oxacyllin resistant strains in white cheese can represent a public health risk since it could serve as a dissemination source, increasing the resistance problem.
Palavras-chave : Staphylococcus spp; oxacyllin resistant; PBP2a protein; white cheese.