Resumo

PARRA GONZALEZ, Evis; CENTENO BRICENO, Sara  e  ARAQUE CALDERON, Yasmina. Antifungal activity of Burkholderia cepacia isolated from yellow corn (Zea mays L.) under different culture conditions. Rev. Soc. Ven. Microbiol. [online]. 2009, vol.29, n.2, pp.103-109. ISSN 1315-2556.

The antifungal activity against phytophatogenic fungi of four Burkholderia cepacia strains isolated from yellow corn (Zea mays L.) from different plantations at the Montes Municipality, Sucre State, Venezuela, was evaluated in vitro. The antagonist activity was compared by using a control strain of environmental origin CVCM 626 (ATCC 25416) and one clinical isolate CVM 1282, with the direct dual confrontation method, and an indirect method with agar-diffusion disks. The tests were done in triplicate, measuring the inhibition halos that occurred, and applying a one way variance analysis, as well the Tukey test. The four isolated strains (SR2, ER3, LG12 and N10) showed similar antifungal activity over Trichoderma viride and produced a total or partial inhibition of sporulation of fungi such as Fusarium moniliforme, Fusarium solani, Aspergillus niger and Penicillium expansum. The largest inhibition halos were produced by the control strain CVCM 626, with a 9,9 mm average. There was no evidence of any antagonism level using the clinical isolate CVCM 1282. Burkholderia cepacia showed an increase of antagonist activity in presence of some carbon sources such as xilose, maltose, mannose, and fructose and of amino-acids such as cystine, valine and leucine, in an acid pH range (4 to 5.5), and a 35^oC temperature.

Palavras-chave : antifungal activity; Burkholderia cepacia; phytopathogenic fungi.

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