Resumo

PONCE DE LEON, Patricia; VALVERDE, Juana  e  RIQUELME, Bibiana. Biorheological activity of Ascaris lumbricoides extracts in human erythrocytes. Rev. Soc. Ven. Microbiol. [online]. 2012, vol.32, n.1, pp.62-69. ISSN 1315-2556.

Previous studies have reported that A. lumbricoides extracts capture sialic acid from human erythrocytes, modifying their charge. The purpose was to evaluate in vitro the possible biorheological alterations due to the effect of extracts from adult parasites. The activity of 8 extracts over Group O erythrocytes was analyzed incubating the globular sediment with an equal volume of extract and with PBS. The distribution and morphology of the aggregates was determined (Digital Image Analysis), percent of charge (Alcian Blue method), aggregation kinetics (erythroaggregameter), and visco-elasticity (erythroderformeter). The results showed that all the extracts produce a decrease of the percentage of isolated cells and an increase of the size of aggregates, without altering their morphology. Red cells treated with 6 extracts mediated a significant alteration of their superficial charge and with 5 of them the aggregation velocity was less than that of the control. A lineal correlation between the kinetic parameters and the protein concentration of the extracts occurred. One of the treatments modified the fluidity of the membrane and two decreased erythrocyte deformability, but none of them affected elasticity. These in vitro experiences allowed concluding that contact of the extracts with the erythrocytes produces hemorheological alterations

Palavras-chave : Ascaris lumbricoides; biorheological activity; human erythrocytes; sialic acid.

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