Abstract

TEDESCO-MAIULLARI, Rosa María  and  GUEVARA, Armando. Molecular epidemiology of extended spectrum ß-lactamases producing Klebsiella pneumoniae. Rev. Soc. Ven. Microbiol. [online]. 2012, vol.32, n.2, pp.101-106. ISSN 1315-2556.

The rapid emergence of antimicrobial resistance to extended spectrum ß-lactamases (ESBL) has a significant clinical impact, and is considered a public health problem. The purpose of this study was to determine if there is clonal dissemination of ESBL producer K. pneumonia in adult patients hospitalized at the Complejo Hospitalario “Ruiz y Paez” (CHRP) of Ciudad Bolivar, Venezuela. Thirteen K. pneumoniae strains isolated from adult patients with intra-hospital infections were evaluated. The phenotypic confirmation of ESBL production was done with the double disk synergy and combined disk methods. The molecular typing of the isolates was done through pulse field electrophoresis (PFE). All the strains presented fluoroquinolone-associated resistance and 9 were aminoglucoside resistant. Twelve susceptibility phenotypes were found; phenotype I prevailed in 2 strains. PFE revealed that most of the strains presented different pulsetypes, discarding a possible clonal relationship among them, except for 2 isolates which presented undistinguishable pulsetypes, both from the same hospitalization service. It can be concluded that at the CHRP there was no clonal dissemination of ESBL producer K. pneumoniae during the March-June 2011 period.

Keywords : Klebsiella pneumoniae; extended spectrum ß-lactamase; pulse field electrophoresis.

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