Resumo

ZAMORA, Alejandra Carolina  e  MALAVER, Nora. Methodological aspects for the culture and quantification of heterotrophic sulfate-reducing bacteria. Rev. Soc. Ven. Microbiol. [online]. 2012, vol.32, n.2, pp.121-125. ISSN 1315-2556.

This paper presents a comparison of various methods for culture, quantification, and maintenance of sulfate-reducing bacteria (SRB) under laboratory conditions, using liquid and semisolid media for water and soil samples. Starkey, Postgate B, API and modified Baars media were used with an incubation time of 21 days in a GasPack™ anaerobic jars type. The modified Baars medium was more efficient for the quantification of SRB in both liquid and semisolid media when compared with other culture media tested, detecting differences of three orders of magnitude in soil samples and in two orders for water samples at 8 days of incubation. The semisolid modified Baars medium in Petri dishes allowed the isolation of pure cultures of SRB by the streak plate method. It was found that strains in liquid modified Baars medium remain viable for up to three months, while in the same semisolid medium were kept only one month.

Palavras-chave : hydrogen sulfide; sulfate-reducing bacteria; culture media; culture methods; SRB quantification.

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