Abstract

DEVERA, Rodolfo et al. Use of cultures for the diagnosis of Blastocystis sp.. Rev. Soc. Ven. Microbiol. [online]. 2013, vol.33, n.1, pp.60-65. ISSN 1315-2556.

A study for comparing the yield of cultures by direct examination and by spontaneous sedimentation for the diagnosis of Blastocystis sp. was carried out. Blastocystis sp. was cultured in a modification of the Boeck and Drbohlav medium. The material used corresponded to 100 fecal samples from inhabitants of the Itopoicon indigenous community, Heres municipality, Bolivar State. Of the 100 samples cultured and submitted to direct examination and spontaneous sedimentation, 90 gave a diagnosis of Blastocystis sp. Of these 90, 83 were positive in culture, while 60 were positive at direct examination, and 57 in spontaneous sedimentation. In conclusion, culture showed a higher yield (83%), than direct examination (60%) and spontaneous sedimentation (57%), for the diagnosis of Blastocystis sp.

Keywords : Blastocystis sp; diagnosis; culture.