Resumo

DE GUGLIELMO, Zoraya et al. DNA extraction from paraffin embedded samples without using xylene for HPV detection and typing. Rev. Soc. Ven. Microbiol. [online]. 2013, vol.33, n.1, pp.83-86. ISSN 1315-2556.

In the present study, DNA from cervical biopsies with a cytological report of intraepithelial cervical lesion and embedded in paraffin blocks was extracted using the “QIAamp DNA mini kit” (QIAGEN, Germany), and the Human Papyloma Virus (HPV) was detected. In the original procedure, the xylene incubation was eliminated, therefore reducing the washes of the biological material; the quality of the DNA extracted was tested by PCR for detecting the β-globine gene and HPV, with 84.3% and 28.1% positivity, respectively. These results were comparable to those of other similar studies, and they demonstrate that it is possible to obtain PCR amplifiable cellular DNA from paraffin embedded samples which can be used efficiently for molecular procedures, eliminating xylene toxicity, and decreasing the manipulation of the biological material by reducing washes.

Palavras-chave : paraffin blocks; DNA extraction; PCR; HPV.