Abstract

UZCATEGUI NEGRON, Mayela del Carmen; SERRANO, José Antonio  and  PANIZO, María Mercedes. Culture conditions that enhance the production of antimicrobial substances from pathogenic and soil actinomycetes. Rev. Soc. Ven. Microbiol. [online]. 2013, vol.33, n.2, pp.134-139. ISSN 1315-2556.

The present study evaluated 300 actinomyces strains (140 from clinical samples and 160 from soil samples). The production of antimicrobial substances by the total actinomyces strains was evaluated using the following broths: Bennet (M1), (M2), and malt-dextrose yeast extract broth YMD (M3). Cultures were adjusted to optimal conditions in order to obtain high bioactive metabolite concentrations. The fermentation process was used with incubation periods which varied between 5 and 10 days. The highest bioactive metabolite production was obtained when the M2 medium was used. Antimicrobial activity was favored with the addition of dextrose, proteose peptone, yeast extract, potassium phosphate (K2PO4), magnesium sulfate (MgSO4) x 7H2O and calcium carbonate (CaCO2). Growth patterns, antimicrobial activity, and biomass production of the actinomyces were related to the three culture media used. The strains which showed strong antimicrobial activity against most gram positive and gram negative bacteria and fungi were selected for future studies where the bioactive metabolites produced will be extracted, purified, and characterized.

Keywords : culture media; bioactive metabolites; actinomyces; optimization; antimicrobial activity.

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