Resumo

AMELI MARCOZZI, Gladys Inocencia; GUTIERREZ GARCIA, Cristina del Rosario; D'ANGELO, Pierina  e  RANGEL, Héctor. Optimization of the reverse PCR technique for HIV detection in plasma of infected patients. Rev. Soc. Ven. Microbiol. [online]. 2013, vol.33, n.2, pp.157-161. ISSN 1315-2556.

Even though PCR is the conventional technique for HIV diagnosis in proviral DNA, the qualitative RT-PCR technique could constitute a support tool for HIV diagnosis in infected patients since one of its main advantages is the use of plasma samples instead of whole blood, which facilitates its transportation from the various regions of the country. The main objective of this study was the evaluation of the optimal conditions regarding use of primers and reverse transcriptase enzyme, for the synthesis of complementary DNA (RT-PCR) of the HIV sheath in plasma samples of HIV positive patients. The results obtained in this study suggest that the use of random primers, in a reactive mixture with the RT “SuperScript” enzyme TM (200 U/mL), allows maximizing the amplifying efficiency of the HIV-1 sheath gene, as compared with specific primers. This offers a methodological alternative using plasma samples for HIV detection in those persons who due to various reasons cannot travel to the reference institution.

Palavras-chave : reverse transcription; polymerase chain reaction; HIV; random primer; patient plasma.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )