Abstract

ACUNA RAMIREZ, Saraí del Valle; SANCHEZ, Elia  and  ABADIA-PATINO, Lorena. Typification of methicillin-resistance in Staphylococcus spp. strains isolated from University Hospital “Antonio Patricio de Alcalᔠin Cumana, Sucre State, Venezuela. Rev. Soc. Ven. Microbiol. [online]. 2014, vol.34, n.1, pp.4-9. ISSN 1315-2556.

Staphylococcus spp. figures as one of the most important bacteria for human beings. The molecular characterization of methicillin-resistant Sttaphylococcus strains (MRS) is necessary for knowing what type of staphylococcal chromosomic complex transporting the mec complex (SCCmec) circulates in a certain area. Twenty one methicillin-resistant S. aureus and 29 coagulase negative (CNS) Staphylococcus strains isolated at the Bacteriology Laboratory of the HUAPA were studied during the January-July 2007 period. The study included antibiogram, multiple PCR, and minimum inhibitory concentration (MIC) determination of the mecA positive strains; 19 S. aureus and 24 CNS strains were oxacilline resistant, correlated with the presence of the mec.A gene, mainly in S. aureus since some discordances were observed with the CNS strains. Only Staphylococcus SCCmec type I and II were identified. The SCCmec characterization identified the Staphylococcus type circulating in the hospital, possibilitating the monitoring of future changes of these strains.

Keywords : Staphylococcus; mec.A; methicillin; SCCmec.

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