Resumo

RODRIGUEZ ROJAS, Hilmarys; MENDOZA, Mireya; CORREA, Doryanna  e  CASARES, Elsy. Evaluación morfológica y bioquímica de aislados clínicos de Trichophyton sppMorphological and biochemical evaluation of clinical isolates of Trichophyton spp. Rev. Soc. Ven. Microbiol. [online]. 2015, vol.35, n.1, pp.31-34. ISSN 1315-2556.

Due to the pleomorphism and macroscopic variability of the Trichophyton genus, identification methods based solely on the morphological characteristics are insufficient for its classification. The objective was to evaluate the morphological and biochemical characteristics of clinical isolates of Trichophyton spp. The study included 98 isolates previously identified by macro and micromorphology that were reassessed by means of microculture sheet, in vitro hair perforation, urea hydrolysis and culture on Trichophyton and BCP-MS-G agars. For morphological analysis, urea hydrolysis tests and in vitro hair perforation results were: T. rubrum 51%, T. mentagrophytes 24%, T. interdigitale 16.6%, T. tonsurans 7% and Trichophyton spp. 2%. The urea hydrolysis test showed 9.4% error. Culture on the BCP-MS-G medium allowed to differentiate T. rubrum cottony A type from T. interdigitale and the identification of Trichophyton spp. as T. rubrum granular B type. The agreement between the previous assessment and reassessment was 70% and when morphophysiological and BCP-MS-G culture were considered, agreement reached 96%. There was 30% overdiagnosis for T. rubrum. Cultures on BCP-MS-G agar are suggested to differentiate T. interdigitale from T. rubrum A type and other biotypes of T. rubrum B type.

Palavras-chave : dermatophytes; Trichophyton spp; identification; BCP-MS-G medium; Trichophyton agars.

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