Resumo

BERRIZBEITIA, Mariolga et al. Standardization, validation and application of indirect immunofluorescence assay in an epidemiological study of Trypanosoma cruzi infection . Rev. Soc. Ven. Microbiol. [online]. 2015, vol.35, n.1, pp.45-50. ISSN 1315-2556.

An indirect immunofluorescence assay (IIF) was standardized using fixed epimastigotes of T. cruzi (isolate MHOM/VE/08/AU) and after validation was applied to determine the seroepidemiology of T. cruzi infection in Rio Brito (Sucre State). The parasites were grown in liver infusion tryptose and fixed with formaldehyde (2%). To validate, the test 49 confirmed as positive and 50 as negative for infection sera were used. To assess cross-reactions 48 sera from patients with confirmed cutaneous leishmaniasis, toxoplasmosis and helminthiasis were analyzed. The IIF test was used to evaluate 50 blood samples collected on filter paper, from Rio Brito, Sucre state population. The epidemiological risk factors were determined by Fisher’s exact test and the odds ratio. The IIF was 100% sensitive and 93.87% specific. The seroprevalence of infection in the community of Rio Brito was 16%. The variables associated with infection were the recognition of the vector and its presence around the houses. The seroprevalence of this infection is high in Rio Brito, where there are epidemiological variables to maintain the cycle of the parasite. 

Palavras-chave : inmunofluorescencia indirecta; seroprevalencia; Trypanosoma cruzi; enfermedad de Chagas; población rural; Venezuela; indirect immunofluorescence; seroprevalence; Trypanosoma cruzi; Chagas disease; rural population; Venezuela.

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